Design of reactive porous polymer supports for high throughput bioreactors: Poly(2-vinyl-4;4 dimethylazlactone-co-acrylamide-co-ethylene dimethacrylate) monoliths

Shaofeng Xie, Frantisek Svec, Jean M J Fréchet*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

174 Scopus citations

Abstract

Enzymatic bioreactors with both high flow characteristics and mechanical stability based on macroporous poly(2-vinyl-4,4-dimethylazlactone-co-acry) amide-co-ethylene dimethacrylate) monoliths have been prepared. Covalent immobilization of trypsin on these support is achieved in a single reaction step using the azlactone functional groups. Optimization of hydrophilic/hydrophobic properties of the monolith affords a support that does not shrink in water and leads to immobilized enzyme that shows high activity in the hydrolysis of both low and high molecular weight substrates such as L-benzoyl arginine ethyl ester and casein. The catalytic activity of the monolithic reactor is maintained even at a flow velocity of 180 cm/min, which substantially exceeds those reported in the literature for packed bed reactors.

Original languageEnglish (US)
Pages (from-to)30-35
Number of pages6
JournalBiotechnology and Bioengineering
Volume62
Issue number1
DOIs
StatePublished - Jan 5 1999
Externally publishedYes

Keywords

  • Azlactone
  • Immobilized enzyme
  • Monolith
  • Polymer support
  • Trypsin

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

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