Design and validation of a highly discriminatory 10-locus Y-chromosome STR multiplex system

María Eugenia D'Amato, Vladimir B. Bajic, Sean P. Davison

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The Y-chromosome STRs (short tandem repeat) markers are routinely utilized in the resolution of forensic casework related to sexual assault. For this, the forensic community has adopted a set of eleven (core) Y-STR that is incorporated in all commercial diagnostic systems. Our previous studies of Y-STR polymorphisms in the South African population identified low levels of diversity and discrimination capacity for many commercial marker sets, determining a limited applicability of these systems to the local population groups. To overcome this shortcoming, we designed a Y-STR 10-plex system that shows higher discriminatory capacity (DC) than available commercial systems. The markers were selected from a population group of 283 individuals with African, European and Asian ancestry genotyped at 45 Y-STRs, applying an optimization based selection procedure to achieve the highest possible DC with the minimal number of markers. The 10-plex was satisfactorily subjected to developmental validation tests following the SWGDAM guidelines and shows potential for its application to genealogical and evolutionary studies. © 2010 Elsevier Ireland Ltd.
Original languageEnglish (US)
Pages (from-to)122-125
Number of pages4
JournalForensic Science International: Genetics
Volume5
Issue number2
DOIs
StatePublished - Mar 2011

ASJC Scopus subject areas

  • Genetics
  • Pathology and Forensic Medicine

Fingerprint

Dive into the research topics of 'Design and validation of a highly discriminatory 10-locus Y-chromosome STR multiplex system'. Together they form a unique fingerprint.

Cite this