Depth-of-field extension and 3D reconstruction in digital holographic microscopy

Isabelle Bergöend*, Tristan Colomb, Nicolas Pavillon, Yves Emery, Christian Depeursinge

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

15 Scopus citations

Abstract

The limited depth-of-field is a main drawback of microscopy that prevents from observing, for example, thick semi-transparent objects with all their features in focus. Several algorithms have been developed during the past years to fuse images having various planes of focus and thus obtain a completely focused image with virtually extended depth-of-field. We present a comparison of several of these methods in the particular field of digital holographic microscopy, taking advantage of some of the main properties of holography. We especially study the extended depth-of-field for phase images reconstructed from the hologram of a biological specimen. A criterion of spatial measurement on the object is considered, completed with a visual criterion. The step of distance taken into account to build the stack of images is less than the instrument depth-of-field. Then, preserving the distance of focus associated with each pixel of the image, a three-dimensional representation is presented after automatic detection of the object. The limits of such a method of extraction of 3D information are discussed.

Original languageEnglish (US)
Title of host publicationModeling Aspects in Optical Metrology II
DOIs
StatePublished - 2009
Externally publishedYes
EventModeling Aspects in Optical Metrology II - Munich, Germany
Duration: Jun 15 2009Jun 16 2009

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume7390
ISSN (Print)0277-786X

Other

OtherModeling Aspects in Optical Metrology II
CountryGermany
CityMunich
Period06/15/0906/16/09

Keywords

  • 3D reconstruction
  • Depth-of-field extension
  • Digital holographic microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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