DeepGO: predicting protein functions from sequence and interactions using a deep ontology-aware classifier

Maxat Kulmanov, Ameer Mohammed Asif Khan, Robert Hoehndorf

Research output: Contribution to journalArticlepeer-review

113 Scopus citations

Abstract

Motivation A large number of protein sequences are becoming available through the application of novel high-throughput sequencing technologies. Experimental functional characterization of these proteins is time-consuming and expensive, and is often only done rigorously for few selected model organisms. Computational function prediction approaches have been suggested to fill this gap. The functions of proteins are classified using the Gene Ontology (GO), which contains over 40 000 classes. Additionally, proteins have multiple functions, making function prediction a large-scale, multi-class, multi-label problem. Results We have developed a novel method to predict protein function from sequence. We use deep learning to learn features from protein sequences as well as a cross-species protein–protein interaction network. Our approach specifically outputs information in the structure of the GO and utilizes the dependencies between GO classes as background information to construct a deep learning model. We evaluate our method using the standards established by the Computational Assessment of Function Annotation (CAFA) and demonstrate a significant improvement over baseline methods such as BLAST, in particular for predicting cellular locations.
Original languageEnglish (US)
Pages (from-to)660-668
Number of pages9
JournalBioinformatics
Volume34
Issue number4
DOIs
StatePublished - Sep 27 2017

Fingerprint

Dive into the research topics of 'DeepGO: predicting protein functions from sequence and interactions using a deep ontology-aware classifier'. Together they form a unique fingerprint.

Cite this