De novo generation of plant centromeres at tandem repeats

Chee How Teo, Inna Lermontova, Andreas Houben, Michael Mette, Ingo Schubert*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Artificial minichromosomes are highly desirable tools for basic research, breeding, and biotechnology purposes. We present an option to generate plant artificial minichromosomes via de novo engineering of plant centromeres in Arabidopsis thaliana by targeting kinetochore proteins to tandem repeat arrays at non-centromeric positions. We employed the bacterial lactose repressor/lactose operator system to guide derivatives of the centromeric histone H3 variant cenH3 to LacO operator sequences. Tethering of cenH3 to non-centromeric loci led to de novo assembly of kinetochore proteins and to dicentric carrier chromosomes which potentially form anaphase bridges. This approach will be further developed and may contribute to generating minichromosomes from preselected genomic regions, potentially even in a diploid background.

Original languageEnglish (US)
Pages (from-to)233-241
Number of pages9
JournalChromosoma
Volume122
Issue number3
DOIs
StatePublished - Jan 1 2013

Keywords

  • De novo centromere formation
  • Kinetochore proteins
  • Lac operator system
  • Lac repressor
  • Technical advance
  • cenH3 targeting

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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