Background Artificial neural networks (ANNs) are a robust class of machine learning models and are a frequent choice for solving classification problems. However, determining the structure of the ANNs is not trivial as a large number of weights (connection links) may lead to overfitting the training data. Although several ANN pruning algorithms have been proposed for the simplification of ANNs, these algorithms are not able to efficiently cope with intricate ANN structures required for complex classification problems. Methods We developed DANNP, a web-based tool, that implements parallelized versions of several ANN pruning algorithms. The DANNP tool uses a modified version of the Fast Compressed Neural Network software implemented in C++ to considerably enhance the running time of the ANN pruning algorithms we implemented. In addition to the performance evaluation of the pruned ANNs, we systematically compared the set of features that remained in the pruned ANN with those obtained by different state-of-the-art feature selection (FS) methods. Results Although the ANN pruning algorithms are not entirely parallelizable, DANNP was able to speed up the ANN pruning up to eight times on a 32-core machine, compared to the serial implementations. To assess the impact of the ANN pruning by DANNP tool, we used 16 datasets from different domains. In eight out of the 16 datasets, DANNP significantly reduced the number of weights by 70%–99%, while maintaining a competitive or better model performance compared to the unpruned ANN. Finally, we used a naïve Bayes classifier derived with the features selected as a byproduct of the ANN pruning and demonstrated that its accuracy is comparable to those obtained by the classifiers trained with the features selected by several state-of-the-art FS methods. The FS ranking methodology proposed in this study allows the users to identify the most discriminant features of the problem at hand. To the best of our knowledge, DANNP (publicly available at www.cbrc.kaust.edu.sa/dannp) is the only available and on-line accessible tool that provides multiple parallelized ANN pruning options. Datasets and DANNP code can be obtained at www.cbrc.kaust.edu.sa/dannp/data.php and https://doi.org/10.5281/zenodo.1001086.