Cytoplasmic calcium stimulates exocytosis in a plant secretory cell

Mark Tester*, Robert Zorec

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

Although exocytosis is likely to occur in plant cells, the control of this process is the subject of speculation, as no direct measurements of vesicle fusion to the plasma membrane have been made. We used the patch clamp technique to monitor the secretory activity of single aleurone protoplasts by measuring membrane capacitance (Cm), while dialyzing the cytosol with different Ca2+ containing solutions. Secretory activity increased with [Ca2+]i ∼ 1 μM. This demonstrates directly the existence of exocytosis in plant cells, and suggests that both plant and animal cells share common mechanisms (cytosolic Ca2+) for the control of exocytotic secretion.

Original languageEnglish (US)
Pages (from-to)864-867
Number of pages4
JournalBiophysical Journal
Volume63
Issue number3
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Biophysics

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