Cyclic mononucleotides modulate potassium and calcium flux responses to H2O2 in Arabidopsis roots

Natalia Maria Ordoñez, Claudius Marondedze, Ludivine Thomas, Stefania Pasqualini, Lana Shabala, Sergey Shabala, Christoph A Gehring

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Cyclic mononucleotides are messengers in plant stress responses. Here we show that hydrogen peroxide (H2O2) induces rapid net K+-efflux and Ca2+-influx in Arabidopsis roots. Pre-treatment with either 10 μM cAMP or cGMP for 1 or 24 h does significantly reduce net K+-leakage and Ca2+-influx, and in the case of the K+-fluxes, the cell permeant cyclic mononucleotides are more effective. We also examined the effect of 10 μM of the cell permeant 8-Br-cGMP on the Arabidopsis microsomal proteome and noted a specific increase in proteins with a role in stress responses and ion transport, suggesting that cGMP is sufficient to directly and/or indirectly induce complex adaptive changes to cellular stresses induced by H2O2. © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Original languageEnglish (US)
Pages (from-to)1008-1015
Number of pages8
JournalFEBS Letters
Volume588
Issue number6
DOIs
StatePublished - Feb 13 2014

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Biophysics

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