Cryopreservation of human brain tissue allowing timely production of viable adult human brain cells for autologous transplantation

Jean François Brunet*, Luc Pellerin, Pierre Magistretti, Jean Guy Villemure

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Background. Autologous transplantation is an attractive approach to treat some neurological diseases. A major obstacle is the capacity to produce cells for transplantation at the appropriate time. We describe a cryopreservation procedure for adult human brain tissue allowing the generation of cells in vitro. Methods. Neurological resections were dissected to separate white and grey matter. Fractions were frozen in a specific cryopreservation medium containing a selected serum and stored in liquid nitrogen. Tissue was thawed, cells were mechanically dissociated, expanded in culture and characterized by immunochemistry. Results. Adult human brain tissue cryopreserved for up to two years was successfully used to generate brain cells that could be maintained in culture for up to 100 days. Cells expressed a variety of neuroectodermal markers including GFAP, S100β, and neurofilament. Conclusion. A successful procedure for cryopreservation of adult human brain tissue has been established that might facilitate future autologous transplantation strategies.

Original languageEnglish (US)
Pages (from-to)179-183
Number of pages5
JournalCryobiology
Volume47
Issue number2
DOIs
StatePublished - Oct 2003
Externally publishedYes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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