CRISPR-Based Directed Evolution for Crop Improvement.

Haroon Butt, Syed Shan-e-Ali Zaidi, Norhan Hassan, Magdy M. Mahfouz

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Directed evolution involves generating diverse sequence variants of a gene of interest to produce a desirable trait under selective pressure. CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9) systems can be programmed to target any genomic locus and perform targeted directed evolution. Here, we discuss the opportunities and challenges of this emerging platform for targeted crop improvement.
Original languageEnglish (US)
JournalTrends in biotechnology
DOIs
StatePublished - Aug 30 2019

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