CO2 assimilation in the chemocline of Lake Cadagno is dominated by a few types of phototrophic purple sulfur bacteria

Nicola Storelli, Sandro Peduzzi, Maged Saad, Niels Ulrik Frigaard, Xavier Perret, Mauro Tonolla*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Lake Cadagno is characterized by a compact chemocline that harbors high concentrations of various phototrophic sulfur bacteria. Four strains representing the numerically most abundant populations in the chemocline were tested in dialysis bags in situ for their ability to fix CO2. The purple sulfur bacterium Candidatus 'Thiodictyon syntrophicum' strain Cad16T had the highest CO2 assimilation rate in the light of the four strains tested and had a high CO2 assimilation rate even in the dark. The CO2 assimilation of the population represented by strain Cad16T was estimated to be up to 25% of the total primary production in the chemocline. Pure cultures of strain Cad16T exposed to cycles of 12 h of light and 12 h of darkness exhibited the highest CO2 assimilation during the first 4 h of light. The draft genome sequence of Cad16T showed the presence of cbbL and cbbM genes, which encode form I and form II of RuBisCO, respectively. Transcription analyses confirmed that, whereas cbbM remained poorly expressed throughout light and dark exposure, cbbL expression varied during the light-dark cycle and was affected by the available carbon sources. Interestingly, the peaks in cbbL expression did not correlate with the peaks in CO2 assimilation.

Original languageEnglish (US)
Pages (from-to)421-432
Number of pages12
JournalFEMS Microbiology Ecology
Volume84
Issue number2
DOIs
StatePublished - May 1 2013

Keywords

  • Candidatus 'Thiodictyon syntrophicum' sp. nov. strain Cad16
  • CbbL and cbbM mRNA
  • Primary production
  • RuBisCO

ASJC Scopus subject areas

  • Microbiology
  • Ecology
  • Applied Microbiology and Biotechnology

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