Conjugative transfer of resistance determinants among human and bovine Streptococcus agalactiae

Tatiana Castro Abreu Pinto, Natália Silva Costa, Ana Beatriz de Almeida Corrêa, Ivi Cristina Menezes de Oliveira, Marcos Correa de Mattos, Alexandre Soares Rosado, Leslie Claude Benchetrit

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Streptococcus agalactiae (GBS) is a major source of human perinatal diseases and bovine mastitis. Erythromycin (Ery) and tetracycline (Tet) are usually employed for preventing human and bovine infections although resistance to such agents has become common among GBS strains. Ery and Tet resistance genes are usually carried by conjugative transposons (CTns) belonging to the Tn916 family, but their presence and transferability among GBS strains have not been totally explored. Here we evaluated the presence of Tet resistance genes (tetMand tetO) and CTns among Ery-resistant (Ery-R) and Ery-susceptible (Ery-S) GBS strains isolated from human and bovine sources; and analyzed the ability for transferring resistance determinants between strains from both origins. Tet resistance and int-Tn genes were more common among Ery-R when compared to Ery-S isolates. Conjugative transfer of all resistance genes detected among the GBS strains included in this study (ermA, ermB, mef, tetM and tetO), in frequencies between 1.10−7 and 9.10−7, was possible from bovine donor strains to human recipient strain, but not the other way around. This is, to our knowledge, the first report of in vitro conjugation of Ery and Tet resistance genes among GBS strains recovered from different hosts.
Original languageEnglish (US)
Pages (from-to)785-789
Number of pages5
JournalBrazilian Journal of Microbiology
Volume45
Issue number3
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

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