Confocal imaging of ionised calcium in living plant cells

D. A. Williams*, S. H. Cody, Christoph Gehring, R. W. Parish, P. J. Harris

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Laser-scanning confocal microscopy has been used in conjunction with Fluo-3, a highly fluorescent visible wavelength probe for Ca2+, to visualise Ca2+-dynamics in the function of living plant cells. This combination has overcome many of the problems that have limited the use of fluorescence imaging techniques in the study of the role of cations (Ca2+ and H+) in plant cell physiology and enables these processes to be studied in single cells within intact plant tissue preparations. Maize coleoptiles respond to application of ionophores and plant growth hormones with elevations in cytosolic Ca2+ that can be resolved with a high degree of spatial resolution and can be interpreted quantitatively.

Original languageEnglish (US)
Pages (from-to)291-297
Number of pages7
JournalCell Calcium
Volume11
Issue number4
DOIs
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

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