Confirmation of hepatitis C virus transmission through needlestick accidents by molecular evolutionary analysis

Kaoru Suzuki, Masashi Mizokami, Johnson Y.N. Lau*, Naoto Mizoguchi, Koji Kato, Yoshiki Mizuno, Takeshi Sodeyama, Kendo Kiyosawa, Takashi Gojobori

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

To document the transmission of hepatitis C virus (HCY) through needlestick accidents, 3 health workers who acquired HCY through such accidents and their HCY donor patients were studied using molecular evolutionary analysis based on the HCY E2 region. At least six clones were sequenced from each subject. Nucleotide substitutions were estimated by the six-parameter method, and a phylogenetic tree was constructed by the neighbor-joining method. HCY isolates from the donor patient and the recipient were nested in one monophyletic cluster; this clustering was confirmed to be statistically significant by bootstrap analysis. The nucleotide divergence among the isolates from the recipient was always smaller than that from the donor, supporting the notion that the direction of transmission was from the donor to the recipient. These findings provide evidence, at a molecular evolutionary level, that HCV was transmitted through needlestick accidents.

Original languageEnglish (US)
Pages (from-to)1575-1578
Number of pages4
JournalJournal of Infectious Diseases
Volume170
Issue number6
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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