Computational analysis and functional expression of ancestral copepod luciferase

Yasuhiro Takenaka*, Akiko Noda-Ogura, Tadashi Imanishi, Atsushi Yamaguchi, Takashi Gojobori, Yasushi Shigeri

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

We recently reported the cDNA sequences of 11 copepod luciferases from the superfamily Augaptiloidea in the order Calanoida. They were classified into two groups, Metridinidae and Heterorhabdidae/Lucicutiidae families, by phylogenetic analyses. To elucidate the evolutionary processes, we have now further isolated 12 copepod luciferases from Augaptiloidea species ( Metridia asymmetrica, Metridia curticauda, Pleuromamma scutullata, Pleuromamma xiphias, Lucicutia ovaliformis and Heterorhabdus tanneri). Codon-based synonymous/nonsynonymous tests of positive selection for 25 identified copepod luciferases suggested that positive Darwinian selection operated in the evolution of Heterorhabdidae luciferases, whereas two types of Metridinidae luciferases had diversified via neutral mechanism. By in silico analysis of the decoded amino acid sequences of 25 copepod luciferases, we inferred two protein sequences as ancestral copepod luciferases. They were expressed in HEK293 cells where they exhibited notable luciferase activity both in intracellular lysates and cultured media, indicating that the luciferase activity was established before evolutionary diversification of these copepod species.

Original languageEnglish (US)
Pages (from-to)201-205
Number of pages5
JournalGene
Volume528
Issue number2
DOIs
StatePublished - Oct 10 2013

Keywords

  • Ancestral gene
  • Bioluminescence
  • Coelenterazine
  • Copepod
  • Luciferase
  • Luciferin

ASJC Scopus subject areas

  • Genetics

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