Complete meiosis from human induced pluripotent stem cells

C. Eguizabal, N. Montserrat, R. Vassena, M. Barragan, E. Garreta, L. Garcia-Quevedo, F. Vidal, A. Giorgetti, A. Veiga, J. C. Izpisua Belmonte*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

136 Scopus citations

Abstract

Gamete failure-derived infertility affects millions of people worldwide; for many patients, gamete donation by unrelated donors is the only available treatment. Embryonic stem cells (ESCs) can differentiate in vitro into germ-like cells, but they are genetically unrelated to the patient. Using an in vitro protocol that aims at recapitulating development, we have achieved, for the first time, complete differentiation of human induced pluripotent stem cells (hiPSCs) to postmeiotic cells. Unlike previous reports using human ESCs, postmeiotic cells arose without the over-expression of germ-line related transcription factors. Moreover, we consistently obtained haploid cells from hiPSCs of different origin (keratinocytes and cord blood), produced with a different number of transcription factors, and of both genetic sexes, suggesting the independence of our approach from the epigenetic memory of the reprogrammed somatic cells. Our work brings us closer to the production of personalized human gametes in vitro.

Original languageEnglish (US)
Pages (from-to)1186-1195
Number of pages10
JournalStem Cells
Volume29
Issue number8
DOIs
StatePublished - Aug 1 2011

Keywords

  • Germ cells
  • Haploid cells
  • Human induced pluripotent stem cells
  • In vitro differentiation
  • Meiosis

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

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