Comparative study of performances of lipase immobilized asymmetric polysulfone and polyether sulfone membranes in olive oil hydrolysis

S. Gupta, Yogesh Singh, S. Javiya, M. Bhambi, C. S. Pundir, K. Singh, A. Bhattacharya*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

In the present study, lipase was immobilized via glutaraldehyde crosslinking on the polysulfone and polyether sulfone asymmetric membranes. The results indicated that the overall immobilization of lipase is related to the hydrophobicity of the membrane material and thus higher immobilization is achieved for polysulfone membrane. The evidence of immobilization is done by XRD, SEM, contact angle and porometric studies. Hydrolytic activity of lipase in immobilized form is determined by hydrolyzing olive oil and compared with hydrolytic activity of free lipase. The effect of different reaction parameters viz., temperature, pH, substrate concentration, and incubation time on the lipase activity is investigated. The observed maximum reaction rate (Vmax) and Michaelis-Menten constant (Km) of polysulfone and polyether sulfone is determined.

Original languageEnglish (US)
Pages (from-to)145-151
Number of pages7
JournalInternational Journal of Biological Macromolecules
Volume42
Issue number2
DOIs
StatePublished - Mar 1 2008

Keywords

  • Hydrophobicity
  • Immobilization
  • Lipase
  • Polyether sulfone
  • Polysulfone

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Comparative study of performances of lipase immobilized asymmetric polysulfone and polyether sulfone membranes in olive oil hydrolysis'. Together they form a unique fingerprint.

Cite this