Comparative proteome analysis of 3T3-L1 adipocyte differentiation using iTRAQ-coupled 2D LC-MS/MS

Feng Ye, Huoming Zhang, Yi Xuan Yang, Huai Dong Hu, Siu Kwan Sze, Wei Meng, Jingru Qian, Hong Ren, Bao Lin Yang, Ming Ying Luo, Xiaoqiong Wu, Wu Zhu, Wei Jun Cai*, Jian Bin Tong

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    32 Scopus citations

    Abstract

    Adipose tissue is critical in obesity and type II diabetes. Blocking of adipocyte differentiation is one of the anti-obesity strategies targeting on strong rise in fat storage and secretion of adipokine(s). However, the molecular basis of adipocyte differentiation and its regulation remains obscure. Therefore, we exposed 3T3-L1 cell line to appropriate hormonal inducers as adipocyte differentiation model. Using iTRAQ-coupled 2D LC-MS/MS, a successfully exploited high-throughput proteomic technology, we nearly quantitated 1,000 protein species and found 106 significantly altered proteins during adipocyte differentiation. The great majority of differentially expressed proteins were related to metabolism enzymes, structural molecules, and proteins involved in signal transduction. In addition to previously reported differentially expressed molecules, more than 20 altered proteins previously unknown to be involved with adipogenic process were firstly revealed (e.g., HEXB, DPP7, PTTG1IP, PRDX5, EPDR1, SPNB2, STEAP3, TPP1, etc.). The partially differential proteins were verified by Western blot and/or real-time PCR analysis. Furthermore, the association of PCX and VDAC2, two altered proteins, with adipocyte conversion was analyzed using siRNA method, and the results showed that they could contribute considerably to adipogenesis. In conclusion, our data provide valuable information for further understanding of adipogenesis.

    Original languageEnglish (US)
    Pages (from-to)3002-3014
    Number of pages13
    JournalJournal of Cellular Biochemistry
    Volume112
    Issue number10
    DOIs
    StatePublished - Oct 1 2011

    Keywords

    • 3T3-L1 adipocytes
    • differentiation
    • iTRAQ
    • proteomics

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Fingerprint

    Dive into the research topics of 'Comparative proteome analysis of 3T3-L1 adipocyte differentiation using iTRAQ-coupled 2D LC-MS/MS'. Together they form a unique fingerprint.

    Cite this