Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens

Yousuke Nishio, Yoji Nakamura, Yutaka Kawarabayasi, Yoshihiro Usuda, Eiichiro Kimura, Shinichi Sugimoto, Kazuhiko Matsui, Akihiko Yamagishi, Hisashi Kikuchi, Kazuho Ikeo, Takashi Gojobori*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

176 Scopus citations

Abstract

Corynebacterium efficiens is the closest relative of Corynebacterium glutamicum, a species widely used for the industrial production of amino acids. C. efficiens but not C. glutamicum can grow above 40°C. We sequenced the complete C efficiens genome to investigate the basis of its thermostability by comparing its genome with that of C. glutamicum. The difference in GC content between the species was reflected in codon usage and nucleotide substitutions. Our comparative genomic study clearly showed that there was tremendous bias in amino acid substitutions in all orthologous ORFs. Analysis of the direction of the amino acid substitutions suggested that three substitutions are important for the stability of the C. efficiens proteins: from lysine to arginine, serine to alanine, and serine to threonine. Our results strongly suggest that the accumulation of these three types of amino acid substitutions correlates with the acquisition of thermostability and is responsible for the greater GC content of C. efficiens.

Original languageEnglish (US)
Pages (from-to)1572-1579
Number of pages8
JournalGenome Research
Volume13
Issue number7
DOIs
StatePublished - Jul 1 2003

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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