Combined laser trapping and small-angle X-ray scattering experiment for the study of liposome colloidal microparticles

Dan Cojoc*, Enrico Ferrari, Valeria Garbin, Enzo Di Fabrizio, Heinz Amenitsch, Michel Rappolt, Barbara Sartori, Christian Riekel, Manfred Burghammer

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We present and discuss a new experimental setup to perform small angle X-ray scattering and diffraction (SAXSD) of localized liposome colloidal microparticles. A home-built inverted infrared laser tweezers microscope is used to trap, manipulate and aggregate micron-scale liposome particles at single locations inside a 100 microns glass capillary. The micro-focused X-ray and the laser beams are aligned to intersect each other perpendiculary, allowing to associate the X-ray diffraction signal to the micron-sized region of interest inside the capillary. Throughout the laser tweezer setup, using diffractive optical elements implemented on a spatial light modulator, we are able to manipulate small aggregates of colloidal particles (liposomes) and fix them in the optical path of the X-ray beam. We present and discuss first scattering and diffraction experiments on phospholipid liposomes, at the ID 13 microfocus beamline of the European Synchrotron Radiation Facility (ESRF The results demonstrate that we can push the limit of measurable cluster size close to a single liposome.

Original languageEnglish (US)
Title of host publicationOptical Trapping and Optical Micromanipulation III
Volume6326
DOIs
StatePublished - 2006
Externally publishedYes
EventOptical Trapping and Optical Micromanipulation III - San Diego, CA, United States
Duration: Aug 13 2006Aug 17 2006

Other

OtherOptical Trapping and Optical Micromanipulation III
CountryUnited States
CitySan Diego, CA
Period08/13/0608/17/06

Keywords

  • Optical tweezers
  • Phospholipids
  • Single biological entity
  • X-ray microdiffraction

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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