Chiral electrochromatography with a 'moulded' rigid monolithic capillary column

Eric C. Peters*, Kevin Lewandowski, Miroslav Petro, Frantisek Svec, Jean M.J. Fréchet

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

128 Scopus citations

Abstract

Several monolithic chiral stationary phases for reversed-phase electrochromatography have been prepared within the confines of untreated fused silica capillaries by the direct copolymerization of the chiral monomer 2-hydroxyethyl methacrylate (N-L-valine-3,5-dimethylanilide) carbamate with ethylene dimethacrylate, 2-acrylamido-2-methyl-1-propanesulfonic acid and butyl or glycidyl methacrylate in the presence of a porogenic solvent. The hydrophilicity of the stationary phase, which may be enhanced further by the hydrolysis of the epoxide functionalities of the glycidyl methacrylate moieties within the monolith, was found to have a pronounced effect on the enantioseparation. Using the most hydrophilic monolithic capillary column and optimized elution conditions, a separation of N-(3,5-dinitrobenzoyl)leucine diallylamide enantiomers with an efficiency of 61000 plates m-1 and a resolution of 2.0 was achieved.

Original languageEnglish (US)
Pages (from-to)83-86
Number of pages4
JournalAnalytical Communications
Volume35
Issue number3
StatePublished - 1998
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Chiral electrochromatography with a 'moulded' rigid monolithic capillary column'. Together they form a unique fingerprint.

Cite this