Chemical Component and Proteomic Study of the Amphibalanus (= Balanus) amphitrite Shell

Gen Zhang, Li-sheng He, Yue-Him Wong, Ying Xu, Yu Zhang, Pei-Yuan Qian

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

As typical biofoulers, barnacles possess hard shells and cause serious biofouling problems. In this study, we analyzed the protein component of the barnacle Amphibalanus (= Balanus) amphitrite shell using gel-based proteomics. The results revealed 52 proteins in the A. Amphitrite shell. Among them, 40 proteins were categorized into 11 functional groups based on KOG database, and the remaining 12 proteins were unknown. Besides the known proteins in barnacle shell (SIPC, carbonic anhydrase and acidic acid matrix protein), we also identified chorion peroxidase, C-type lectin-like domains, serine proteases and proteinase inhibitor proteins in the A. Amphitrite shell. The sequences of these proteins were characterized and their potential functions were discussed. Histology and DAPI staining revealed living cells in the shell, which might secrete the shell proteins identified in this study.
Original languageEnglish (US)
Pages (from-to)e0133866
JournalPLOS ONE
Volume10
Issue number7
DOIs
StatePublished - 2015
Externally publishedYes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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