Characterization of protein-protein interactions: Application to the understanding of peroxisome biogenesis

Sebastien Leon, Ivet Suriapranata, Mingda Yan, Naganand Rayapuram, Amar Patel, Suresh Subramani

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

With the approaching completion of the Pichia pastoris genome, a greater emphasis will have to be placed on the proteome and the protein-protein interactions between its constituents. This chapter discusses methods that have been used for the study of such interactions among both soluble and membrane-associated proteins in peroxisome biogenesis. The procedures are equally applicable to other cellular processes.

Original languageEnglish (US)
Number of pages19
JournalMethods in Molecular Biology
Volume389
DOIs
StatePublished - Aug 8 2007

Keywords

  • Co-immunoprecipitation
  • Mass spectrometry
  • Membrane protein complexes
  • Protein complexes
  • Protein interactions
  • Soluble protein complexes
  • Tandem affinity purification
  • TAP-tag
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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