Characterization and partial purification of entomocin 110, a newly identified bacteriocin from Bacillus thuringiensis subsp. Entomocidus HD110

Ameur Cherif*, Wafa Rezgui, Noura Raddadi, Daniele Daffonchio, Abdellatif Boudabous

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

A new bacteriocin produced by Bacillus thuringiensis subsp. entomocidus was identified. The antibacterial activity termed entomocin 110 was produced starting at mid-logarithmic growth phase, reaching its maximum at the early and during stationary phase. The bacteriocin obtained from culture supernatant was inhibitory to several Gram-positive bacteria including Listeria monocytogenes, Paenibacillus larvae and other Bacillus species. Entomocin 110 was shown to be heat stable and resistant to pH variation and to organic solvents. The inhibitory activity was totally lost after proteinase K treatment, thereby revealing its proteinaceous nature. The mode of action of entomocin 110 was bactericidal and bacteriolytic. Upon partial purification with ammonium sulphate precipitation followed by butanol extraction, an active peptide with an apparent molecular weight of 4.8 kDa was identified. Cross inhibition tests with bacteriocin producer strains and plasmid profiles indicated that entomocin 110 is a new bacteriocin, which genetic determinants are probably harbored by the chromosome.

Original languageEnglish (US)
Pages (from-to)684-692
Number of pages9
JournalMicrobiological Research
Volume163
Issue number6
DOIs
StatePublished - Jan 1 2008

Keywords

  • Bacillus thuringiensis
  • Entomocin 110
  • Heat stable bacteriocin

ASJC Scopus subject areas

  • Microbiology

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