Cellular Dynamics Revealed by Digital Holographic Microscopy☆

P. Marquet, Christian Depeursinge, P. Jourdain

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Digital holographic microscopy (DHM) is a new optical method that provides, without the use of any contrast agent, real-time, three-dimensional images of transparent living cells, with an axial sensitivity of a few tens of nanometers. They result from the hologram numerical reconstruction process, which permits a sub wavelength calculation of the phase shift, produced on the transmitted wave front, by the optically probed cells, namely the quantitative phase signal (QPS). Specifically, in addition to measurements of cellular surface morphometry and intracellular refractive index (RI), various biophysical cellular parameters including dry mass, absolute volume, membrane fluctuations at the nanoscale and biomechanical properties, transmembrane water permeability as swell as current, can be derived from the QPS. This article presents how quantitative phase DHM (QP-DHM) can explored cell dynamics at the nanoscale with a special attention to both the study of neuronal dynamics and the optical resolution of local neuronal network.
Original languageEnglish (US)
Title of host publicationReference Module in Neuroscience and Biobehavioral Psychology
PublisherElsevier BV
Pages675-683
Number of pages9
ISBN (Print)9780128093245
DOIs
StatePublished - Nov 22 2016

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