Cellular Dynamics Revealed by Digital Holographic Microscopy

P. Marquet*, Christian Depeursinge

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Digital holographic microscopy (DHM) is a new optical method taking advantage of coherent properties of light propagation and it provides, without the use of any contrast agent, real-time three-dimensional images of transparent living cells, with an axial sensitivity of a few tens of nanometers. Specifically, both three-dimensional images of cellular surface morphometry and intracellular refractive index mapping are provided. They result from the hologram numerical reconstruction process, which permits a subwavelength calculation of the phase shift, produced on the transmitted wavefront, by the optically probed cells. Consequently, DHM offers unique possibilities for monitoring biological processes involving rapid and discrete morphological changes, including membrane nano-movements in specific cellular microdomains.

Original languageEnglish (US)
Title of host publicationEncyclopedia of Neuroscience
PublisherElsevier Ltd
Pages675-683
Number of pages9
ISBN (Print)9780080450469
DOIs
StatePublished - Jan 1 2009

Keywords

  • Cellular dynamics
  • Digital holography
  • Excitotoxocity
  • Glutamate
  • Interferometry
  • Membrane fluctuations
  • Microscopy
  • Neuron cell culture
  • Optical imaging
  • Red blood cells

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Cellular Dynamics Revealed by Digital Holographic Microscopy'. Together they form a unique fingerprint.

Cite this