Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy

Nicolas Pavillon*, Alexander Benke, Daniel Boss, Corinne Moratal, Jonas Kühn, Pascal Jourdain, Christian Depeursinge, Pierre J. Magistrettiy, Pierre Marquety

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell parameters including absolute volume, shape and integral intracellular refractive index. In combination with the numerous different specific fluorescent cellular probes, this multimodality microscopy can address important issues in cell biology. This is demonstrated by the study of intracellular calcium homeostasis associated with the change in cell volume, which play a critical role in the excitotoxicity-induced neuronal death. Comparison between quantitative phase (left) and fluorescence signal (right) on neuronal cellular bodies.

Original languageEnglish (US)
Pages (from-to)432-436
Number of pages5
JournalJournal of Biophotonics
Volume3
Issue number7
DOIs
StatePublished - Jul 1 2010
Externally publishedYes

Keywords

  • Fluorescence microscopy
  • Interference microscopy
  • Optics and photonics
  • cell biology

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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