Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells

Daiji Okamura, Ikuma Maeda, Hirofumi Taniguchi, Yuko Tokitake, Makiko Ikeda, Keiko Ozato, Nathan Mise, Kuniya Abe, Toshiaki Noce, Juan Carlos Izpisua Belmonte, Yasuhisa Matsui*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Transcription elongation is stimulated by positive transcription elongation factor b (P-TEFb), for which activity is repressed in the 7SK small nuclear ribonucleoprotein (7SK snRNP) complex. We show here a critical role of 7SK snRNP in growth control of primordial germ cells (PGCs). The expression of p15INK4b, a cyclin-dependent kinase inhibitor (CDKI) gene, in PGCs is selectively activated by P-TEFb and its recruiting molecule, Brd4, when the amount of active P-TEFb is increased due to reduction of the 7SK snRNP, and PGCs consequently undergo growth arrest. These results indicate that CDKI gene-specific control of transcription by 7SK snRNP plays a pivotal role in the maintenance of PGC proliferation.

Original languageEnglish (US)
Pages (from-to)2477-2482
Number of pages6
JournalGenes and Development
Volume26
Issue number22
DOIs
StatePublished - Nov 15 2012

Keywords

  • 7SK snRNP
  • CDK inhibitor
  • Larp7
  • P-TEFb
  • Primordial germ cell
  • Transcription

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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