Cdc2MsB, a cognate cdc2 gene from alfalfa, complements the G1/S but not the G2/M transition of budding yeast cdc28 mutants

Heribert Hirt*, Aniko Páy, Laszlo Bögre, Irute Meskiene, Erwin Heberle-Bors

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

The product of the cdc2 gene encodes the p34 cdc2 protein kinase that controls entry of yeast cells into S phase and mitosis. In higher eukaryotes, at least two cdc2-like genes appear to be involved in these processes. A cdc2 homologous gene has previously been isolated from alfalfa and shown to complement a fission yeast cdc2 ts mutant. Here the isolation of cdc2MsB, a cognate cdc2gene from alfalfa (Medicago sativa) is reported. Southern blot analysis shows that cdc2MsA and cdc2MsB are present as single copy genes in different tetraploid Medicago species. cdc2MsB encodes a slightly larger mRNA (1.5 kb) than cdc2MsA (1.4 kb). Both genes were found to be expressed at similar steady state levels in different alfalfa organs. Expression levels of both cdc2Ms genes correlate with the proliferative state of the organs. Complementation studies revealed that in contrast to cdc2MsA, cdc2MsB was not able to rescue a cdc2 ts fission yeast mutant. cdc2MsB was also unable to rescue a G2/M-arrested cdc20 ts budding yeast mutant which could be rescued by expression of the cdc2MsA gene. Conversely, cdc2MsB but not cdc2MsA was found to complement the G1/S block of another cdc28 ts budding yeast mutant. These results suggest that cdc2MsA and cdc2MsB function at different control points in the cell cycle.

Original languageEnglish (US)
Pages (from-to)61-69
Number of pages9
JournalPlant Journal
Volume4
Issue number1
DOIs
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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