Capillary electrochromatography in anion-exchange and normal-phase mode using monolithic stationary phases

Michael Lämmerhofer, Frantisek Svec, Jean Frechet*, Wolfgang Lindner

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

109 Scopus citations

Abstract

Hydrophilic macroporous weak and strong anion-exchange stationary phases have been prepared in a monolithic format within untreated fused-silica capillaries by the simple thermally or UV-initiated polymerization of 2-dimethylaminoethyl methacrylate, 2-hydroxyethyl methacrylate and ethylene dimethacrylate in the presence of a binary porogenic mixture of dodecanol and cyclohexanol. The tertiary amino functionalities were then alkylated in situ to afford strong anion-exchangers. These new monolithic stationary phases with optimized porous properties were used for the CEC separation of various organic anions. Thus, a mixture of 2-substituted propionic acid drugs (profens) was separated in 13 min and high column efficiencies of up to 231 000 plates/m were achieved. The separation of substituted benzoic acids indicates that the selectivity results primarily from the anion-exchange interactions, while electrophoretic migration contributes only slightly. In addition, these hydrophilic anion-exchangers are also able to separate weakly acidic, neutral and basic compounds such as phenols, xanthines and aromatic amines in normal-phase electrochromatographic mode.

Original languageEnglish (US)
Pages (from-to)265-277
Number of pages13
JournalJournal of Chromatography A
Volume925
Issue number1-2
DOIs
StatePublished - Aug 3 2001

Keywords

  • Benzoic acids
  • Electrochromatography
  • Monoliths
  • Porous polymers
  • Profens
  • Stationary phases, electrochromatograpy

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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