Butenolide inhibits marine fouling by altering the primary metabolism of three target organisms

Yifan Zhang, Huoming Zhang, Lisheng He, Changdong Liu, Ying Xü, Pei-Yuan Qian

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Butenolide is a very promising antifouling compound that inhibits ship hull fouling by a variety of marine organisms, but its antifouling mechanism was previously unknown. Here we report the first study of butenolides molecular targets in three representative fouling organisms. In the barnacle Balanus (=Amphibalanus) amphitrite, butenolide bound to acetyl-CoA acetyltransferase 1 (ACAT1), which is involved in ketone body metabolism. Both the substrate and the product of ACAT1 increased larval settlement under butenolide treatment, suggesting its functional involvement. In the bryozoan Bugula neritina, butenolide bound to very long chain acyl-CoA dehydrogenase (ACADVL), actin, and glutathione S-transferases (GSTs). ACADVL is the first enzyme in the very long chain fatty acid β-oxidation pathway. The inhibition of this primary pathway for energy production in larvae by butenolide was supported by the finding that alternative energy sources (acetoacetate and pyruvate) increased larval attachment under butenolide treatment. In marine bacterium Vibrio sp. UST020129-010, butenolide bound to succinyl-CoA synthetase β subunit (SCSβ) and inhibited bacterial growth. ACAT1, ACADVL, and SCSβ are all involved in primary metabolism for energy production. These findings suggest that butenolide inhibits fouling by influencing the primary metabolism of target organisms. © 2012 American Chemical Society.
Original languageEnglish (US)
Pages (from-to)1049-1058
Number of pages10
JournalACS Chemical Biology
Volume7
Issue number6
DOIs
StatePublished - Apr 10 2012

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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