Bacterial diversity shift determined by different diets in the gut of the spotted wing fly Drosophila suzukii is primarily reflected on acetic acid bacteria

Violetta Vacchini, Elena Gonella, Elena Crotti, Erica M. Prosdocimi, Fabio Mazzetto, Bessem Chouaia, Matteo Callegari, Francesca Mapelli, Mauro Mandrioli, Alberto Alma, Daniele Daffonchio

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The pivotal role of diet in shaping gut microbiota has been evaluated in different animal models, including insects. Drosophila flies harbour an inconstant microbiota among which acetic acid bacteria (AAB) are important components. Here, we investigated the bacterial and AAB components of the invasive pest Drosophila suzukii microbiota, by studying the same insect population separately grown on fruit-based or non-fruit artificial diet. AAB were highly prevalent in the gut under both diets (90 and 92% infection rates with fruits and artificial diet, respectively). Fluorescent in situ hybridization and recolonization experiments with green fluorescent protein (Gfp)-labelled strains showed AAB capability to massively colonize insect gut. High-throughput sequencing on 16S rRNA gene indicated that the bacterial microbiota of guts fed with the two diets clustered separately. By excluding AAB-related OTUs from the analysis, insect bacterial communities did not cluster separately according to the diet, suggesting that diet-based diversification of the community is primarily reflected on the AAB component of the community. Diet influenced also AAB alpha-diversity, with separate OTU distributions based on diets. High prevalence, localization and massive recolonization, together with AAB clustering behaviour in relation to diet, suggest an AAB role in the D. suzukii gut response to diet modification. This article is protected by copyright. All rights reserved.
Original languageEnglish (US)
Pages (from-to)91-103
Number of pages13
JournalEnvironmental Microbiology Reports
Volume9
Issue number2
DOIs
StatePublished - Mar 13 2017

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: King Abdullah University of Science and Technology supported the study through the baseline research funds to D.D. This work was partially funded by Consorzio di Ricerca Sperimentazione e Divulgazione per l’Ortofrutticoltura Piemontese, within the project “Programma di ricerca, sperimentazione e dimostrazione agricola in frutticoltura e orticoltura – 2014 – Indagini sul nuovo dittero esotico Drosophila suzukii responsabile di gravi danni alle drupacee”. E.C. acknowledges personal support from “Piano Sviluppo di Ateneo: Linea B-Dotazione annuale per attività istituzionale” in the project “Acetic acid bacteria cell factories”.

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