Bacterial community diversity assessment in municipal solid waste compost amended soil using DGGE and ARISA fingerprinting methods

Hanene Cherif*, Hadda Ouzari, Massimo Marzorati, Lorenzo Brusetti, Naceur Jedidi, Abdennaceur Hassen, Daniele Daffonchio

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Bacterial community structure and diversity of Tunisian agricultural soil treated with different amounts of municipal solid waste compost (MSWC) and other fertilizers were studied using DGGE and ARISA fingerprinting methods. Sequence analysis of dominant DGGE bands revealed the presence of three major clusters, Cytophaga/Flexibacter/Bacteroides (CFB) group, Proteobacteria and Acidobacteria group. Using ARISA profiles, dominant populations were assigned to low and high GC Gram positive bacteria, Cyanobacteria, Spirochetes and Cytophagales. The two methods revealed the absence of significant bacterial community shifts related to the different MSWC applications. Moreover, indigenous bacterial population of the used loam-clayey soil was observed to limit proliferation and survival of Proteobacteria, initially dominant in MSWC and farmyard manure. Effectiveness of the two methods for soil bacterial community studying was shown. While DGGE was more accurate for bacterial identification, ARISA was more practical for handling and rapid estimation of dominant bacteria.

Original languageEnglish (US)
Pages (from-to)1159-1167
Number of pages9
JournalWorld Journal of Microbiology and Biotechnology
Volume24
Issue number7
DOIs
StatePublished - Jul 1 2008

Keywords

  • ARISA
  • Bacterial community structure
  • Compost
  • DGGE

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

Fingerprint

Dive into the research topics of 'Bacterial community diversity assessment in municipal solid waste compost amended soil using DGGE and ARISA fingerprinting methods'. Together they form a unique fingerprint.

Cite this