Auxin-binding-protein antibodies and peptides influence stomatal opening and alter cytoplasmic pH

Christoph Gehring, Robyn M. McConchie, Michael A. Venis, Roger W. Parish*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Previous work has shown that stomatal opening induced by indole-3-acetic acid (IAA) in epidermal strips of the orchid Paphiopedilum tonsum L. is preceded by a reduction in cytoplasmic pH (pHi) of the guard cells. We now report that Fab fragments of an auxin-agonist antibody (D16), directed against a putative auxin-binding domain of the auxin-binding protein ABP1, induce stomatal opening and decrease guard-cell pH(i), as monitored with the acetomethoxy ester of the ratiometric pH indicator Snarf-1. Similar activity was shown by a monoclonal antibody against the same domain. The C-terminal dodecapeptide, Pz152-163 of maize ABP1 (ABPzm1) induced guard-cell alkalinization and closed stomata, as did Fab fragments of a monoclonal antibody (MAC 256) recognising the C-terminal region of ABPzm1. By implicating, for the first time, an auxin-binding protein in mediation of an auxin-dependent physiological response, these findings strongly support an auxin-receptor role for ABP1.

Original languageEnglish (US)
Pages (from-to)581-586
Number of pages6
JournalPlanta
Volume205
Issue number4
DOIs
StatePublished - Aug 1 1998

Keywords

  • Alkalinization
  • Auxin receptor
  • Cytoplasmic pH
  • Guard cell
  • Paphiopedilum
  • Stomatal opening

ASJC Scopus subject areas

  • Genetics
  • Plant Science

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