Assessing the impact of synthetic estrogen on the microbiome of aerated submerged fixed-film reactors simulating tertiary sewage treatment and isolation of estrogen-degrading consortium: Science of the Total Environment

C.L.S. Vilela, R.S. Peixoto, C.T.C.D.C. Rachid, J.P. Bassin

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

17α-ethinylestradiol (EE2) is a synthetic estrogen that can cause harmful effects on animals, such as male feminization and infertility. However, the impact of the EE2 contamination on microbial communities and the potential role of bacterial strains as bioremediation agents are underexplored. The aim of this work was to evaluate the impact of EE2 on the microbial community dynamics of aerated submerged fixed-film reactors (ASFFR) simulating a polishing step downstream of a secondary sewage treatment. For this purpose, the reactors were fed with a synthetic medium with low COD content (around 50 mg l−1), supplemented (reactor H) or not (reactor C) with 1 μg l−1 of EE2. Sludge samples were periodically collected during the bioreactors operation to assess the bacterial profile over time by 16S rRNA gene amplicon sequencing or by bacterial isolation using culture-dependent approach. The results revealed that the most abundant phyla in both reactors were Proteobacteria and Bacteroidetes. At genus level, Chitinophagaceae, Nitrosomonas and Bdellovibrio predominated. Significant effects caused by EE2 treatment and bioreactors operating time were observed by non-metric multidimensional scaling. Therefore, even at low concentrations as 1 μg l−1, EE2 is capable of influencing the bioreactor microbiome. Culture-dependent methods showed that six bacterial isolates, closely related to Pseudomonas and Acinetobacter genera, could grow on EE2 as the sole carbon source under aerobic conditions. These organisms may potentially be used for the assembly of an EE2-degrading bacterial consortium and further exploited for bioremediation applications, including tertiary sewage treatment to remove hormone-related compounds not metabolized in secondary depuration stages. © 2020 Elsevier B.V.
Original languageEnglish
JournalSci. Total Environ.
Volume743
DOIs
StatePublished - 2020

Keywords

  • 17α-ethinylestradiol
  • ASFFR
  • Bacterial consortium
  • Bioremediation
  • Endocrine disruptors
  • Estrogen contamination
  • Bioconversion
  • Bioreactors
  • Biotechnology
  • Microorganisms
  • RNA
  • Bacterial isolation
  • Bioremediation agent
  • Fixed film reactor
  • Microbial communities
  • Microbial community dynamics
  • Non-metric multidimensional scaling
  • Synthetic estrogens
  • Sewage treatment
  • estrogen
  • ethinylestradiol
  • RNA 16S
  • estradiol derivative
  • chemical oxygen demand
  • community dynamics
  • estrogenic compound
  • gene expression
  • microbial community
  • pollutant removal
  • sewage treatment
  • Acinetobacter
  • aeration
  • amplicon
  • Article
  • bacterium culture
  • bacterium isolation
  • Bacteroidetes
  • Bdellovibrio
  • Chitinophagaceae
  • concentration (parameter)
  • controlled study
  • gene sequence
  • microbial consortium
  • Nitrosomonas
  • nonhuman
  • nucleotide sequence
  • phylum
  • population abundance
  • priority journal
  • Proteobacteria
  • Pseudomonas
  • sludge
  • animal
  • bioreactor
  • male
  • microflora
  • sewage
  • Animalia
  • Bacteria (microorganisms)
  • Animals
  • Estradiol Congeners
  • Estrogens
  • Ethinyl Estradiol
  • Male
  • Microbiota
  • RNA, Ribosomal, 16S
  • Sewage

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