Assessing the Diversity of Marine Bacterial β-Glucosidases by Capillary Electrophoresis Zymography

Jesus Arrieta, Gerhard J. Herndl*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

We propose a new method for the fast separation and detection of β-glucosidases in environmental samples. With this approach, β-glucosidases extracted from bacteria are evidenced by substrate-incorporated capillary electrophoresis (CE zymography) and their kinetic parameters can be determined by repeated injections using different substrate concentrations. Preliminary results obtained with natural bacterial communities from the coastal North Sea suggest that the diversity of β-glucosidases in the marine environment might be much higher than previously observed.

Original languageEnglish (US)
Pages (from-to)4896-4900
Number of pages5
JournalApplied and Environmental Microbiology
Volume67
Issue number10
DOIs
StatePublished - Oct 1 2001

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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