Assessing protein conformational sampling methods based on bivariate lag-distributions of backbone angles

Mehdi Maadooliat, Xin Gao, Jianhua Z. Huang

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Despite considerable progress in the past decades, protein structure prediction remains one of the major unsolved problems in computational biology. Angular-sampling-based methods have been extensively studied recently due to their ability to capture the continuous conformational space of protein structures. The literature has focused on using a variety of parametric models of the sequential dependencies between angle pairs along the protein chains. In this article, we present a thorough review of angular-sampling-based methods by assessing three main questions: What is the best distribution type to model the protein angles? What is a reasonable number of components in a mixture model that should be considered to accurately parameterize the joint distribution of the angles? and What is the order of the local sequence-structure dependency that should be considered by a prediction method? We assess the model fits for different methods using bivariate lag-distributions of the dihedral/planar angles. Moreover, the main information across the lags can be extracted using a technique called Lag singular value decomposition (LagSVD), which considers the joint distribution of the dihedral/planar angles over different lags using a nonparametric approach and monitors the behavior of the lag-distribution of the angles using singular value decomposition. As a result, we developed graphical tools and numerical measurements to compare and evaluate the performance of different model fits. Furthermore, we developed a web-tool (http://www.stat.tamu. edu/~madoliat/LagSVD) that can be used to produce informative animations. © The Author 2012. Published by Oxford University Press.
Original languageEnglish (US)
Pages (from-to)724-736
Number of pages13
JournalBriefings in Bioinformatics
Volume14
Issue number6
DOIs
StatePublished - Aug 27 2012

ASJC Scopus subject areas

  • Molecular Biology
  • Information Systems

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