Assembly of photosynthetic reaction center with ABA tri-block polymersomes: highlights on the protein localization.

Roberto Rocco Tangorra, Alessandra Operamolla, Francesco Milano, Omar Hassan Omar, John Henrard, Roberto Comparelli, Francesca Italiano, Angela Agostiano, Vincenzo De Leo, Roberto Marotta, Andrea Falqui, Gianluca Farinola, Massimo Trotta

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The reconstitution of the integral membrane protein photosynthetic reaction center (RC) in polymersomes, i. e. artificial closed vesicles, was achieved by the micelle-to-vesicle transition technique, a very mild protocol based on size exclusion chromatography often used to drive the incorporation of proteins contemporarily to liposomes formation. An optimized protocol was used to successfully reconstitute the protein in a fully active state in polymersomes formed by the tri-block copolymers PMOXA22-PDMS61-PMOXA22. The RC is very sensitive to its solubilizing environment and was used to probe the positioning of the protein in the vesicles. According to charge-recombination experiments and to the enzymatic activity assay, the RC is found to accommodate in the PMOXA22 region of the polymersome, facing the water bulk solution, rather than in the PDMS61 transmembrane-like region. Furthermore, polymersomes were found to preserve protein integrity efficiently as the biomimetic lipid bilayers but show a much longer temporal stability than lipid based vesicles.
Original languageEnglish (US)
Pages (from-to)1844-1852
Number of pages9
JournalPhotochem. Photobiol. Sci.
Volume14
Issue number10
DOIs
StatePublished - 2015

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