Archaeal and bacterial diversity in acidic to circumneutral hot springs in the Philippines

Qiuyuan Huang, Hongchen Jiang, Brandon R. Briggs, Shang Wang, Weiguo Hou, Gaoyuan Li, Geng Wu, Ramonito Solis, Carlo A. Arcilla, Teofilo Abrajano, Hailiang Dong*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    39 Scopus citations

    Abstract

    The microbial diversity was investigated in sediments of six acidic to circumneutral hot springs (Temperature: 60-92 °C, pH 3.72-6.58) in the Philippines using an integrated approach that included geochemistry and 16S rRNA gene pyrosequencing. Both bacterial and archaeal abundances were lower in high-temperature springs than in moderate-temperature ones. Overall, the archaeal community consisted of sequence reads that exhibited a high similarity (nucleotide identity > 92%) to phyla Crenarchaeota, Euryarchaeota, and unclassified Archaea. The bacterial community was composed of sequence reads moderately related (nucleotide identity > 90%) to 17 phyla, with Aquificae and Firmicutes being dominant. These phylogenetic groups were correlated with environmental conditions such as temperature, dissolved sulfate and calcium concentrations in spring water, and sediment properties including total nitrogen, pyrite, and elemental sulfur. Based on the phylogenetic inference, sulfur metabolisms appear to be key physiological functions in these hot springs. Sulfobacillus (within phylum Firmicutes) along with members within Sulfolobales were abundant in two high-temperature springs (> 76 °C), and they were hypothesized to play an important role in regulating the sulfur cycling under high-temperature conditions. The results of this study improve our understanding of microbial diversity and community composition in acidic to circumneutral terrestrial hot springs and their relationships with geochemical conditions.

    Original languageEnglish (US)
    Pages (from-to)452-464
    Number of pages13
    JournalFEMS Microbiology Ecology
    Volume85
    Issue number3
    DOIs
    StatePublished - Sep 1 2013

    Keywords

    • Biodiversity
    • Hot springs
    • Philippines
    • Pyrosequencing
    • Thermoacidophiles

    ASJC Scopus subject areas

    • Microbiology
    • Ecology
    • Applied Microbiology and Biotechnology

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