Arabidopsis Yak1 protein (AtYak1) is a dual specificity protein kinase

Dongjin Kim, Valentine Otang Ntui, Nianshu Zhang, Liming Xiong

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Yak1 is a member of dual-specificity Tyr phosphorylation-regulated kinases (DYRKs) that are evolutionarily conserved. The downstream targets of Yak1 and their functions are largely unknown. Here, a homologous protein AtYAK1 was identified in Arabidopsis thaliana and the phosphoprotein profiles of the wild type and an atyak1 mutant were compared on two-dimensional gel following Pro-Q Diamond phosphoprotein gel staining. Annexin1, Annexin2 and RBD were phosphorylated at serine/ threonine residues by the AtYak1 kinase. Annexin1, Annexin2 and Annexin4 were also phosphorylated at tyrosine residues. Our study demonstrated that AtYak1 is a dual specificity protein kinase in Arabidopsis that may regulate the phosphorylation status of the annexin family proteins.
Original languageEnglish (US)
Pages (from-to)3321-3327
Number of pages7
JournalFEBS Letters
Volume589
Issue number21
DOIs
StatePublished - Oct 9 2015

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Biophysics

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