ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development

Liesbeth Vercruyssen, Aurine Verkest, Nathalie Gonzalez, Ken S. Heyndrickx, Dominique Eeckhout, Soon Ki Han, Teddy Jégu, Rafal Archacki, Jelle Van Leene, Megan Andriankaja, Stefanie De Bodt, Thomas Abeel, Frederik Coppens, Stijn Dhondt, Liesbeth De Milde, Mattias Vermeersch, Katrien Maleux, Kris Gevaert, Andrzej Jerzmanowski, Moussa BenhamedDoris Wagner, Klaas Vandepoele, Geert De Jaeger, Dirk Inzé*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

121 Scopus citations

Abstract

The transcriptional coactivator ANGUSTIFOLIA3 (AN3) stimulates cell proliferation during Arabidopsis thaliana leaf development, but the molecular mechanism is largely unknown. Here, we show that inducible nuclear localization of AN3 during initial leaf growth results in differential expression of important transcriptional regulators, including GROWTH REGULATING FACTORs (GRFs). Chromatin purification further revealed the presence of AN3 at the loci of GRF5, GRF6, CYTOKININ RESPONSE FACTOR2, CONSTANS-LIKE5 (COL5), HECATE1 (HEC1), and ARABIDOPSIS RESPONSE REGULATOR4 (ARR4). Tandem affinity purification of protein complexes using AN3 as bait identified plant SWITCH/SUCROSE NONFERMENTING (SWI/SNF) chromatin remodeling complexes formed around the ATPases BRAHMA (BRM) or SPLAYED. Moreover, SWI/SNF ASSOCIATED PROTEIN 73B (SWP73B) is recruited by AN3 to the promoters of GRF5, GRF3, COL5, and ARR4, and both SWP73B and BRM occupy the HEC1 promoter. Furthermore, we show that AN3 and BRM genetically interact. The data indicate that AN3 associates with chromatin remodelers to regulate transcription. In addition, modification of SWI3C expression levels increases leaf size, underlining the importance of chromatin dynamics for growth regulation. Our results place the SWI/SNF-AN3 module as a major player at the transition from cell proliferation to cell differentiation in a developing leaf.

Original languageEnglish (US)
Pages (from-to)210-229
Number of pages20
JournalPlant Cell
Volume26
Issue number1
DOIs
StatePublished - Jan 1 2014

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Fingerprint

Dive into the research topics of 'ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development'. Together they form a unique fingerprint.

Cite this