Analyzing Lysosome-Related Organelles by Electron Microscopy

Ilse Hurbain, Maryse Romao, Ptissam Bergam, Xavier Heiligenstein, Graça Raposo

    Research output: Chapter in Book/Report/Conference proceedingChapter

    15 Scopus citations

    Abstract

    Intracellular organelles have a particular morphological signature that can only be appreciated by ultrastructural analysis at the electron microscopy level. Optical imaging and associated methodologies allow to explore organelle localization and their dynamics at the cellular level. Deciphering the biogenesis and functions of lysosomes and lysosome-related organelles (LROs) and their dysfunctions requires their visualization and detailed characterization at high resolution by electron microscopy. Here, we provide detailed protocols for studying LROs by transmission electron microscopy. While conventional electron microscopy and its recent improvements is the method of choice to investigate organelle morphology, immunoelectron microscopy allows to localize organelle components and description of their molecular make up qualitatively and quantitatively.
    Original languageEnglish (US)
    Title of host publicationMethods in Molecular Biology
    PublisherSpringer Nature
    Pages43-71
    Number of pages29
    ISBN (Print)9781493969326
    DOIs
    StatePublished - Apr 30 2017

    ASJC Scopus subject areas

    • Genetics
    • Molecular Biology

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