Analysis of flanking sequences from Dissociation insertion lines: A database for reverse genetics in Arabidopsis

Serguei Parinov, Mayalagu Sevugan, De Ye, Wei Cai Yang, Kumaran Kuppusamy Mande, Venkatesan Sundaresan*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    255 Scopus citations

    Abstract

    We have generated Dissociation (Ds) element insertions throughout the Arabidopsis genome as a means of random mutagenesis. Here, we present the molecular analysis of genomic sequences that flank the Ds insertions of 931 independent transposant lines. Flanking sequences from 511 lines proved to be identical or homologous to DNA or protein sequences in public databases, and disruptions within known or putative genes were indicated for 354 lines. Because a significant portion (45%) of the insertions occurred within sequences defined by GenBank BAC and P1 clones, we were able to assess the distribution of Ds insertions throughout the genome. We discovered a significant preference for Ds transposition to the regions adjacent to nucleolus organizer regions on chromosomes 2 and 4. Otherwise, the mapped insertions appeared to be evenly dispersed throughout the genome. For any given gene, insertions preferentially occurred at the 5' end, although disruption was clearly possible at any intragenic position. The insertion sites of >500 lines that could be characterized by reference to public databases are presented in a tabular format at http://www.plantcell.org/cgi/content/full/11/12/2263/DC1. This database should be of value to researchers using reverse genetics approaches to determine gene function.

    Original languageEnglish (US)
    Pages (from-to)2263-2270
    Number of pages8
    JournalPlant Cell
    Volume11
    Issue number12
    DOIs
    StatePublished - Dec 1 1999

    ASJC Scopus subject areas

    • Plant Science
    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry
    • Cell Biology

    Fingerprint Dive into the research topics of 'Analysis of flanking sequences from Dissociation insertion lines: A database for reverse genetics in Arabidopsis'. Together they form a unique fingerprint.

    Cite this