Analysis of de novo Golgi complex formation after enzyme-based inactivation

Florence Jollivet, Graça Raposo, Ariane Dimitrov, Rachid Sougrat, Bruno Goud, Franck Perez*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    15 Scopus citations

    Abstract

    The Golgi complex is characterized by its unique morphology of closely apposed flattened cisternae that persists despite the large quantity of lipids and proteins that transit bidirectionally. Whether such a structure is maintained through endoplasmic reticulum (ER)-based recycling and auto-organization or whether it depends on a permanent Golgi structure is strongly debated. To further study Golgi maintenance in interphase cells, we developed a method allowing for a drug-free inactivation of Golgi dynamics and function in living cells. After Golgi inactivation, a new Golgi-like structure, containing only certain Golgi markers and newly synthesized cargos, was produced. However, this structure did not acquire a normal Golgi architecture and was unable to ensure a normal trafficking activity. This suggests an integrative model for Golgi maintenance in interphase where the ER is able to autonomously produce Golgi-like structures that need pre-existing Golgi complexes to be organized as morphologically normal and active Golgi elements.

    Original languageEnglish (US)
    Pages (from-to)4637-4647
    Number of pages11
    JournalMolecular Biology of the Cell
    Volume18
    Issue number11
    DOIs
    StatePublished - Nov 1 2007

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics
    • Cell Biology

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