Analysis of cellular structure and dynamics with digital holographic microscopy

P. Marquet*, B. Rappaz, F. Charrière, Y. Emery, C. Depeursinge, P. Magistretti

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

9 Scopus citations

Abstract

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically, the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We discuss some approaches allowing to directly obtain separate measurements of the thickness and the refractive index (RI) of a given living cell from the phase signal.

Original languageEnglish (US)
Title of host publicationBiophotonics 2007
Subtitle of host publicationOptics in Life Science
PublisherSPIE
ISBN (Print)0819467774, 9780819467775
DOIs
StatePublished - Jan 1 2007
EventBiophotonics 2007: Optics in Life Science - Munich, Germany
Duration: Jun 18 2007Jun 20 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6633
ISSN (Print)1605-7422

Other

OtherBiophotonics 2007: Optics in Life Science
CountryGermany
CityMunich
Period06/18/0706/20/07

Keywords

  • Cell imaging
  • Cell morphology
  • Digital holography
  • Refractive index

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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