Analysis of cellular structure and dynamics with digital holographic microscopy

P. Marquet, B. Rappaz, F. Charrière, Y. Emery, C. Depeursinge, P. Magistretti

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically, the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We discuss some approaches allowing to directly obtain separate measurements of the thickness and the refractive index (RI) of a given living cell from the phase signal.

Original languageEnglish (US)
Title of host publicationEuropean Conference on Biomedical Optics, ECBO 2007
PublisherOptical Society of America
ISBN (Print)9780819467713
StatePublished - 2007
Externally publishedYes
EventEuropean Conference on Biomedical Optics, ECBO 2007 - Munich, Germany
Duration: Jun 17 2007Jun 17 2007

Other

OtherEuropean Conference on Biomedical Optics, ECBO 2007
CountryGermany
CityMunich
Period06/17/0706/17/07

Keywords

  • Cell imaging
  • Cell morphology
  • Digital holography
  • Refractive index

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

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