An atlas of RNA base pairs involving modified nucleobases with optimal geometries and accurate energies

Mohit Chawla, R. Oliva, J. M. Bujnicki, Luigi Cavallo

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Posttranscriptional modifications greatly enhance the chemical information of RNA molecules, contributing to explain the diversity of their structures and functions. A significant fraction of RNA experimental structures available to date present modified nucleobases, with half of them being involved in H-bonding interactions with other bases, i.e. ‘modified base pairs’. Herein we present a systematic investigation of modified base pairs, in the context of experimental RNA structures. To this end, we first compiled an atlas of experimentally observed modified base pairs, for which we recorded occurrences and structural context. Then, for each base pair, we selected a representative for subsequent quantum mechanics calculations, to find out its optimal geometry and interaction energy. Our structural analyses show that most of the modified base pairs are non Watson–Crick like and are involved in RNA tertiary structure motifs. In addition, quantum mechanics calculations quantify and provide a rationale for the impact of the different modifications on the geometry and stability of the base pairs they participate in.
Original languageEnglish (US)
Pages (from-to)6714-6729
Number of pages16
JournalNucleic Acids Research
Volume43
Issue number14
DOIs
StatePublished - Jun 27 2015

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