An Arabidopsis thaliana leucine-rich repeat protein harbors an adenylyl cyclase catalytic center and affects responses to pathogens

Chantal Bianchet, Aloysius Tze Wong, Mara Quaglia, May Majed Alquraishi, Christoph A Gehring, Vardis Ntoukakis, Stefania Pasqualini

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Adenylyl cyclases (ACs) catalyze the formation of the second messenger cAMP from ATP. Here we report the characterization of an Arabidopsis thaliana leucine-rich repeat (LRR) protein (At3g14460; AtLRRAC1) as an adenylyl cyclase. Using an AC-specific search motif supported by computational assessments of protein models we identify an AC catalytic center within the N-terminus and demonstrate that AtLRRAC1 can generate cAMP in vitro. Knock-out mutants of AtLRRAC1 have compromised immune responses to the biotrophic fungus Golovinomyces orontii and the hemibiotrophic bacteria Pseudomonas syringae, but not against the necrotrophic fungus Botrytis cinerea. These findings are consistent with a role of cAMP-dependent pathways in the defense against biotrophic and hemibiotrophic plant pathogens.
Original languageEnglish (US)
Pages (from-to)12-22
Number of pages11
JournalJournal of Plant Physiology
Volume232
DOIs
StatePublished - Nov 3 2018

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