An arabidopsis clathrin assembly protein with a predicted role in plant defense can function as an adenylate cyclase

Patience Chatukuta, Tshegofatso B. Dikobe, David T. Kawadza, Katlego S. Sehlabane, Mutsa M. Takundwa, Aloysius Wong, Christoph Gehring, Oziniel Ruzvidzo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Adenylate cyclases (ACs), much like guanylate cyclases (GCs), are increasingly recognized as essential parts of many plant processes including biotic and abiotic stress responses. In order to identify novel ACs, we have applied a search motif derived from experimentally tested GCs and identified a number of Arabidopsis thaliana candidates including a clathrin assembly protein (AT1G68110; AtClAP). AtClAP contains a catalytic centre that can complement the AC-deficient mutant cyaA in E. coli, and a recombinant AtClAP fragment (AtClAP 261–379 ) can produce cyclic adenosine 3′,5′ monophosphate (cAMP) from adenosine triphosphate (ATP) in vitro. Furthermore, an integrated analysis of gene expression and expression correlation implicate cAMP in pathogen defense and in actin cytoskeletal remodeling during endocytic internalization.

Original languageEnglish (US)
Article number15
JournalBiomolecules
Volume8
Issue number2
DOIs
StatePublished - Jun 1 2018

Keywords

  • Adenylate cyclase
  • Arabidopsis thaliana
  • CAMP
  • Clathrin assembly
  • Endocytosis
  • Pathogen responses

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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