Absolute expression values for mouse transcripts: Re-annotation of the READ expression database by the use of CAGE and EST sequence tags

Rimantas Kodzius, Yonehiro Matsumura, Takeya Kasukawa, Kazuro Shimokawa, Shiro Fukuda, Toshiyuki Shiraki, Mari Nakamura, Takahiro Arakawa, Daisuke Sasaki, Jun Kawai, Matthias Harbers, Piero Carninci*, Yoshihide Hayashizaki

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The RIKEN expression array database (READ) provides comprehensive gene expression data for the mouse, which were obtained as relative values from microarray double-staining experiments with E17.5 mRNA as common reference. To assign absolute expression values for mouse transcripts within READ, we applied the E17.5 reference sample to CAGE (cap analysis of gene expression) and expressed sequence tag (EST) high-throughput tag sequencing. Newly assigned values within the READ database were validated by comparison to expression data from serial analysis of gene expression, CAGE and EST experiments. These experiments confirmed the great significance of the absolute expression values within the improved READ database.

Original languageEnglish (US)
Pages (from-to)22-26
Number of pages5
JournalFEBS Letters
Volume559
Issue number1-3
DOIs
StatePublished - Feb 13 2004

Keywords

  • Absolute value
  • Cap analysis of gene expression
  • Expressed sequence tag count
  • Gene expression
  • Microarray
  • Serial analysis of gene expression

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Absolute expression values for mouse transcripts: Re-annotation of the READ expression database by the use of CAGE and EST sequence tags'. Together they form a unique fingerprint.

Cite this